Evolution of translational elongation factor (EF) sequences: reliability of global phylogenies inferred from EF-1 alpha(Tu) and EF-2(G) proteins.
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of Mycobacterium leprae elongation factor (EF-Tu) gene.
The elongation factor EF-Tu is essential in bacterial translation and has sequences which are highly conserved even in phylogenetically distant bacteria. This allowed us to show that Gram negative bacteria had two copies of the tuf gene whereas most Gram positive bacteria including Mycobacteria had one copy of this gene (1). The agent of leprosy, Mycobacterium leprae, has been isolated from nat...
متن کاملPrediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins.
The EF-hand protein with a helix-loop-helix Ca(2+) binding motif constitutes one of the largest protein families and is involved in numerous biological processes. To facilitate the understanding of the role of Ca(2+) in biological systems using genomic information, we report, herein, our improvement on the pattern search method for the identification of EF-hand and EF-like Ca(2+)-binding protei...
متن کاملCloning and nucleotide sequence of a tobacco chloroplast translational elongation factor, EF-Tu.
Translational elongation factor is an essential component for protein synthesis that functions by binding aminoacylated tRNAs to the ribosome-mRNA complex. This function is performed by EF-Tu in prokaryotes and eukaryotic organelles, and by EF-la in eukaryotes (for review see Miller and Weissbach, 1977). In lower photosynthetic eukaryotes, including Chlamydomonas (Baldauf and Palmer, 1990) and ...
متن کاملEF-hand calcium-binding proteins.
The EF-hand motif is the most common calcium-binding motif found in proteins. Several high-resolution structures containing different metal ions bound to EF-hand sites have given new insight into the modulation of their binding affinities. Recently determined structures of members of several newly identified protein families that contain the EF-hand motif in some of their domains, as well as of...
متن کاملMechanism of elongation factor (EF)-Ts-catalyzed nucleotide exchange in EF-Tu. Contribution of contacts at the guanine base.
Nucleotide exchange in elongation factor Tu (EF-Tu) is catalyzed by elongation factor Ts (EF-Ts). Similarly to other GTP-binding proteins, the structural changes in the P loop and the Mg(2+) binding site are known to be important for nucleotide release from EF-Tu. In the present paper, we determine the contribution of the contacts between helix D of EF-Tu at the base side of the nucleotide and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.8.3255